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human ctss elisa kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology human ctss elisa kit
    Human Ctss Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human ctss elisa kit/product/Elabscience Biotechnology
    Average 90 stars, based on 1 article reviews
    human ctss elisa kit - by Bioz Stars, 2026-03
    90/100 stars

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    Proteolytic enzymes expression levels by HFLS and HFLS-OA stimulated with TNFα or LPS measured using the <t>ELISA</t> method. Expression of proteases measured in cellular lysates using the ELISA method. Cells were stimulated for 24 h with 10 ng/mL of TNFα ( A , C , E ) or LPS ( B , D , F ). Afterward, the proteins were isolated, and MMP3 ( A , B ), CTSK ( C , D ), and <t>CTSS</t> ( E , F ) levels were determined. Data were calculated as % of control ± SEM and analyzed with two-way ANOVA followed by Tukey’s post hoc test. * p < 0.05; ** 0.01 > p > 0.001; *** p < 0.001 vs. control group. # p < 0.05; ## 0.01 > p > 0.001; vs. HFLS-OA stimulated groups. HFLS, human fibroblast-like synoviocytes; HFLS-OA, osteoarthritic human fibroblast-like synoviocytes; TNFα, tumor necrosis factor alpha; LPS, lipopolysaccharide; ELISA, enzyme linked immunosorbent assay; MMP3, matrix metalloprotease 3; CTSK, cathepsin K; CTSS, cathepsin S.
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    Proteolytic enzymes expression levels by HFLS and HFLS-OA stimulated with TNFα or LPS measured using the ELISA method. Expression of proteases measured in cellular lysates using the ELISA method. Cells were stimulated for 24 h with 10 ng/mL of TNFα ( A , C , E ) or LPS ( B , D , F ). Afterward, the proteins were isolated, and MMP3 ( A , B ), CTSK ( C , D ), and CTSS ( E , F ) levels were determined. Data were calculated as % of control ± SEM and analyzed with two-way ANOVA followed by Tukey’s post hoc test. * p < 0.05; ** 0.01 > p > 0.001; *** p < 0.001 vs. control group. # p < 0.05; ## 0.01 > p > 0.001; vs. HFLS-OA stimulated groups. HFLS, human fibroblast-like synoviocytes; HFLS-OA, osteoarthritic human fibroblast-like synoviocytes; TNFα, tumor necrosis factor alpha; LPS, lipopolysaccharide; ELISA, enzyme linked immunosorbent assay; MMP3, matrix metalloprotease 3; CTSK, cathepsin K; CTSS, cathepsin S.

    Journal: International Journal of Molecular Sciences

    Article Title: Inflammation-Driven Secretion Potential Is Upregulated in Osteoarthritic Fibroblast-Like Synoviocytes

    doi: 10.3390/ijms231911817

    Figure Lengend Snippet: Proteolytic enzymes expression levels by HFLS and HFLS-OA stimulated with TNFα or LPS measured using the ELISA method. Expression of proteases measured in cellular lysates using the ELISA method. Cells were stimulated for 24 h with 10 ng/mL of TNFα ( A , C , E ) or LPS ( B , D , F ). Afterward, the proteins were isolated, and MMP3 ( A , B ), CTSK ( C , D ), and CTSS ( E , F ) levels were determined. Data were calculated as % of control ± SEM and analyzed with two-way ANOVA followed by Tukey’s post hoc test. * p < 0.05; ** 0.01 > p > 0.001; *** p < 0.001 vs. control group. # p < 0.05; ## 0.01 > p > 0.001; vs. HFLS-OA stimulated groups. HFLS, human fibroblast-like synoviocytes; HFLS-OA, osteoarthritic human fibroblast-like synoviocytes; TNFα, tumor necrosis factor alpha; LPS, lipopolysaccharide; ELISA, enzyme linked immunosorbent assay; MMP3, matrix metalloprotease 3; CTSK, cathepsin K; CTSS, cathepsin S.

    Article Snippet: The protein levels of MMP3 (Human Total MMP-3 Quantikine ELISA Kit, R&D Systems, Minneapolis, MN, USA), CTSK (Human CTSK ELISA Kit, ELK Biotechnology), CTSS (Human CTSS ELISA Kit, ELK Biotechnology), RARRES1 (Human RARRES ELISA Kit, ELK Biotechnology), RARRES2 (Human CHEM ELISA Kit, ELK Biotechnology), and Dkk-1 (Human DKK1 ELISA Kit, ELK Biotechnology) were measured using commercially available enzyme-linked immunosorbent assay kits according to the manufacturers’ instructions.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Isolation